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The purpose of this paper is to use the adaptive response of erythrocytes to oxidative stress as a method for the enhancement of their stability to cryoconservation factors. The oxidative stress of erythrocytes was induced by ozone introduced in erythrocyte suspension as ozonated saline solution. Ozone was produced with a barrier-type ozonator of our design. The concentration of dissolved ozone in ozonated saline solution (OSS) was measured by spectrophotometric method with device Specord UV VIS according to extinction value on Hartley band. The osmotic erythrocyte brittleness was assessed by hemo-globulin liberation from cells in hypotonic sodium chloride solutions. The obtained results give grounds for the development of a new approach to the enhancement of erythrocyte cryoconservation efficiency and possibly other cells based on ozone technologies. The said approach was conditionally named by us the so-called non-cryoprotective method of cryoconservation efficiency enhancement. It consists in the living system natural resource mobilization under adaptive response to oxidative stress and may be used in addition to available cell protective methods during their cryoconservation.
Key words: erythrocytes, cryoconservation, ozone, osmotic brittleness.
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1. Wagner .., Burnett .., Livesey S.A., Connor J. Red blood cell stabilization reduces the effect of cell density on recovery following cryopreservation. Cryobiology. 2000. Vol. 41. 3. P. 178-194.
2. .., .., .. . . . 2006. . 16. 2. . 68-72.